Investigatory Project In Biology For Class 11 Cbse Pdf Download

The study was performed using four SIV-infected macaques, two ART treated for various ART-administration regimens and two untreated controls. All subjects were SIVmac239-infected and treated with ART from the second week post infection until the study termination day. Monkeys were divided into following four groups: a) ART-treated SIV-infected macaques for 2 months (n = 3, M129-08, M129-09, M129-12); b) ART-treated SIV-infected macaques for 26 months (n = 3, M130-03, M130-07, M130-08); c) SIV-infected macaques without ART treatment (n = 2, M131-03, M131-05); and, d) SIV-naïve monkeys (n = 1, M132-02) as controls.

HIV/SIV RNA levels in the plasma and CD4/CD8 T cell counts were measured at different time points after SIV infection in order to consider effect of SIV infection on tissues and relevant immune status. However, since the goal of this study is to reveal the effect of SIV infection and ART on intestinal tissues while considering relevant immune status, this study did not include the measurements for HIV RNA levels in plasma and CD4/CD8 T cell counts. Also, no tissue staining for HIV-p24 was performed in this studies to correlate the HIV RNA levels in plasma or CD4/CD8 T cell counts with the viral RNA levels in intestinal tissues.

Production of monocyte-derived macrophage using peripheral blood monocytes (A) and ileums (B) derived MNCs in culture for 8 days. After 8 days in culture, macrophages were stained for cell surface markers by flow cytometry. (C) Generation of In vitro Infectious virus. Indirect immunofluorescence assay was used to detect SIV viral protein production in macrophages. Total macrophage population was also stained by Cell Tracker blue CMAC for flow cytometric analysis in order to differentiate macrophages from other cells in culture (D). d2c66b5586